Authors : Jong-Hwan Lim, Hong-Gee Lee, Tae-Won Kim, In-Bae Song, Myoung-Seok Kim, Youn-Hwan Hwang, Byung-Kwon Park and Hyo-In Yun
Abstract: A simple and sensitive Agar well diffusion bioassay with Providencia alcalifaciens ATCC 9886 and Liquid Chromatography/Mass Spectrometric (LC/MS) method for cefquinome in pig plasma was evaluated and validated. The proposed microbiological and LC/MS methods for the determination of cefquinome in plasma showed good inter-assay and intra-assay precision, accuracy and linearity. A microbiological assay and LC/MS assay for the determination of cefquinome yielded statistically identical results (r2 = 0.83). The developed microbiological method in pig plasma has been successfully utilized for pharmacokinetic study of cefquinome in pigs. Following intramuscular injection of cefquinome at 1 mg kg-1 in pigs, it was rapidly absorbed with mean Cmax of 0.40 μg mL-1 at 2.33 h. Thereafter, the plasma concentration of cefquinome was declined with 2.36 h of elimination half-life in pigs.
Jong-Hwan Lim, Hong-Gee Lee, Tae-Won Kim, In-Bae Song, Myoung-Seok Kim, Youn-Hwan Hwang, Byung-Kwon Park and Hyo-In Yun, 2011. Comparison of Microbiological Assay and High-Performance Liquid Chromatography/Mass Spectrometry for the Pharmacokinetics of Cefquinome in Pigs. Agricultural Journal, 6: 374-377.