Authors : Indra Taufik Sahli, Umi Kalsum and Sumarno Reto Prawiro
Abstract: Bacterial Biofilm (BF) is a structural form of a group of microorganisms protected by an extracellular matrix which is called Extracellular Polymeric Substance (EPS). Staphylococcus BF EPS consists of Polysaccharide Intercellular Adhesin (PIA), extracellular-DNA, protein and amyloid fibrils. The purpose of this study was to determine the characterization of S. aureus bacterial BF protein based on molecular weight, hemagglutinin protein and see the response of BF protein polyclonal antibodies to several antigens from S. aureus bacteria. The results of the study of BF protein profiles using SDS-PAGE have a molecular weight of 35, 22.4 and 17 kDa. Protein 22.4 kDa has the highest titer (1/4) in the hemagglutination test using mice erythrocytes, so, it is continued to make polyclonal antibodies. BF protein antibodies of 22.4 kDa S. aureus can respond to several antigens from S. aureus bacteria which have a molecular weight of 120, 63, 48, 30 and 22.4 kDa. BF protein S. aureus compossed polymer protein and as can hemagglutinin protein.
Indra Taufik Sahli, Umi Kalsum and Sumarno Reto Prawiro, 2019. Characterization Bacterial Biofilm Protein of Staphylococcus aureus. International Journal of Tropical Medicine, 14: 25-32.