Authors : Hari Paraton, Aulanni’am , Erry Gumilar Dachlan, Widjiati Widjiati, I.W. Arsana Wiyasa and Sumarno Retro Prawiro
Abstract: During pregnancy, the fetus requires substantial elements including protein for growth and development. A pro-inflammatory cascade can cause TNFα expression through NF-κB due to the presence of casein which binds to the TLR. Knowing the effect of case in supplementation diet on chorioamniont issue of premature Rattus norvegicus pregnancies by evaluating the expression of TLR-4, NF-κB and TNF-α. The study was a real experimental laboratory with post-test only control group design. The sample using 32 Rattus norvegicus separated into two Groups (G). G1 who received a regular diet of case in 40 g kg–1 BW/day (G1) and compared with G2 which case in supplementation diet 200 g kg–1 BW/day (G2), since, the first day of pregnancy. Chorioamnion specimens taken on the 10, 12 and 14th days of gestation. Immunohistochemical examination was performed and examined using the Remmele Immuno Reactive Score index. The data was analyzed statistically by SPSS25. LR-4 among G1 control group) has an average value of 3.675±2.001 (95% CI: 0.40-7.00) whereas the treatment Group (G2) has 3.037±2.202 (95% CI: 0.20-7.20), there was no significant difference between both groups. NF-κB was 1.862±1.138 (95% CI: 0.60-5.60) in G1 and was not significantly difference (p = 0.055) against G2 (average 2.455±0.608; 95% CI:2.00-4.20). TNFα was also not significantly different between G11.912±0.655 (95% CI:0.00-2.80) against G-2, 3.644±2.795 (95% CI:1.00-11.40). The administration of casein supplemented nutrition to pregnant Rattus norvegicus was not a significant difference in the expression of TLR-4, NF-κB and TNFα in chorioamnion preterm pregnancy.
Hari Paraton, Aulanni’am , Erry Gumilar Dachlan, Widjiati Widjiati, I.W. Arsana Wiyasa and Sumarno Retro Prawiro, 2020. The Effect of Giving Casein on TLR-4, NF-κB and TNF-α Expression Chorioamnion Premature Rattus Norvegicus. International Journal of Tropical Medicine, 15: 66-73.