Abstract: Aflatoxins are natural toxins that contaminate various types of food and feedstuff leading to health risk in both humans and animals. The aim of this study, was to determine prevalence of aflatoxins contamination and their levels in animal (cattle and poultry) feed samples of groundnut cake meal, wheat bran, crushed sorghum (which are used as raw materials) and manufactured ration in Khartoum State, Sudan. The aflatoxins were extracted using Best Food (BF) method, one of the methods approved by (AOAC), then separated and determined by High Performance Liquid Chromatography (HPLC) adopting Photo Diode Array Detection. The study showed that 64.29% of the samples of animal feed were contaminated by aflatoxins at average concentration of 130.63 μg kg-1. The highest contamination occurred in summer (78.95%) followed by autumn (66.67%) and was found least in winter (47.37%). Manufactured ration showed highest value of contamination (87.50%), with a concentration range of 54.41-579.87 μg kg-1 (average concentration of 207.96 μg kg-1), followed by groundnut cake meal with an overall 69.32% contamination rate and contamination range of 24.29-410.62 (average concentration of 131.45 μg kg-1), wheat bran with 63.64% contamination rate showed range of 4.07-79.85 (average concentration of 31.19 μg kg-1) and crushed sorghum with 36.36% contamination rate and contamination range of 5.46-375.81 μg kg-1 (average concentration of 165.65 μg kg-1). Aflatoxin B1 (AfB1) was the most common contaminant followed by Aflatoxin G1 (AfG1), Aflatoxin B2 (AfB2) and lastly Aflatoxin G2 (AfG2).
Amin O. Elzupir , Younis M.H. Younis , M. Himmat Fadul and Abdelrahim M. Elhussein , 2009. Determination of Aflatoxins in Animal Feed in Khartoum State, Sudan. Journal of Animal and Veterinary Advances, 8: 1000-1003.