Authors : Zhikai Zhang, Lixin Xu , Xiaokai Song , Xiangrui Li and Ruofeng Yan
Abstract: Major Sperm Protein (MSP) is one of the important components of nematode sperms and the characters of this protein were widely studied in Caenorhabdit elegans and Ascaris suum. However, no knowledge of homology proteins in Haemonchus contortus was reported. In the present research, the MSP gene of this parasite (HcMSP) was first cloned and characterized. A pair of primer targeted the conserved sequence of nematode MSPs was designed and a partial of HcMSP gene was obtained by reverse transcription PCR (RT-PCR). Then, the 5' and 3' ends of HcMSP gene were cloned by 5' Rapid Amplification of cDNA ends (RACE) and 3' RACE. The full length of cDNA was obtained by overlapping the sequences of 3' and 5' extremities. The Open Reading Frame (ORF) was amplified by RT-PCR and expressed in prokaryotic cell.
Zhikai Zhang, Lixin Xu , Xiaokai Song , Xiangrui Li and Ruofeng Yan, 2013. Characterization of HcMSP, A Novel Gender Specific Gene from Parasitic Nematode Haemonchus contortus. Journal of Animal and Veterinary Advances, 12: 593-599.