Authors : Chao Xu, Song-Kai Feng, Qin Zhang and Miao-Yun Li
Abstract: Brucella, E. coli O157, Staphylococcus aureus, β-Streptococcus, Erysipelothrix rhusiopathiae, Pseudomonas aeruginosa and Bovine viral diarrhea virus, Sheep pox virus, Goat pox virus, Bluetongue virus, foot and mouth disease virus are common pathogens in fur animal. An oligonucleotide microarray able to detect simultaneously the 6 bacteria and 5 viruses is reported in the present study. The assay was highly specific for detecting the 6 bacteria and 5 viruses in single or multiple infections and as few as 100 copies of specific pathogens target fragments were detected successfully. The 320 archived samples were tested by this assay and the results were 100% consistent with previous results based on conventional PCR and sequencing. The assay is appropriate for the screening of 11 pathogens infections in fur animal due to its high throughput, low-cost, high specificity and sensitivity.
Chao Xu, Song-Kai Feng, Qin Zhang and Miao-Yun Li, 2014. Screening of 11 Pathogens in Fur Animal Basesd on Oligonucleotide Microarray. Journal of Animal and Veterinary Advances, 13: 671-677.