Authors : Na Kong
Abstract: The Hemagglutinin (HA1) gene from H5 subtype Avian Influenza Virus (AIV) and mature Chicken Interleukin-18 (mChIL-18) gene were amplified by designing two pairs of primers according to the sequence published on GenBank. The HA1 gene and mChIL-18 gene were inserted into the downstreams of PH promoter and P10 promoter of baculovirus expression plasmid pFastBacTMDual, respectively. The recombinant pFastBacTMdual-mChIL-18-HA1 (rIL18-HA1) was ransformed into DH10Bac competent cells and expressed in sf9 cells by IFA diction. The results showed that the recombinant protein IL18-HA1 (pIL18-HA1) was simultaneouly expressed in the same sf9 cells with IL18 protein and HA1 protein, respectively. It is the foundation for developing a neotype vaccine against AIV.
Na Kong , 2015. Expression of Recombinant Baculovirus Vetor Double-expressing HA1 Gene from H5 Subtype Avian Influenza Virus and Mature Chicken Interleukin-18 Gene. Journal of Animal and Veterinary Advances, 14: 136-139.