Abstract: The current study was sought out to describe the prevalence of bla_SHV producing Klebsiella pneumoniae isolated from surface waters of Hilla River. In the period from January-April 2015, 101 water samples were collected from 7 different locations of Hilla River. The 35 (34.6%) isolates were specified as K. pneumoniae. All isolates were checked for Extended Spectrum β-Lactamase (ESBL) production phenotypically using disk combination method. About 21 (60%) isolates were screen-positive. The antibiotic resistance profiles of ESBL-producing isolates was evaluated using Kirby-Bauer disk diffusion method. The highest rate of resistance was noticed for penicillin antibiotics (ampicillin and piperacillin) with 85.71% and 80.95%, respectively. Carbapenem resistance was identified in 2 isolates of K. pneumoniae, these were checked further by Polymerase Chian Reaction (PCR) method for detection bla_SHV gene, 2 (100%) isolates gave positive result.