Abstract: The purpose of this study is to investigate the knowledge and attitude regarding organ donation among university students in Korea and to identify the factors influencing on organ donation attitude. A survey was carried out among university students in C Province of Korea from November 11 to December 17, 2016. They responded questionnaires of attitude, knowledge and subjectivity regarding organ donation. About 250 questionnaires were collected and 231 questionnaires were analyzed except for the incomplete one. Data were analyzed using t-test, one-way ANOVA, Scheffe test, descriptive analysis, Pearson’s correlation coefficients and multiple regression analysis. The participant’s attitude regarding organ donation according to general characteristics showed statistically differences in age (F = 3.980, p = 0.009), grade (F = 5.810, p = 0.002), major in university (F = 9.590, p<0.001), monthly income (F = 3.559, p = 0.015), blood donation experience (t = -2.398, p = 0.017), educational experience on organ donation (t = 3.623, p<0.001) and subjectivity on organ donation (F = 7.727, p<0.001). The mean score of attitude regarding organ donation of university students was 64.37±8.43 point (range 18-90), the lowest score was 35 point and the highest score was 87 point. Attitude regarding organ donation showed a significant positive correlation with the knowledge to organ donation (r = 0.313, p<0.001) and the intention to organ donation (r = 0.680, p<0.001). Participant’s attitude regarding organ donation was affected significantly by utilitarian life-seeking (β = 0.741, t = 17.093, p<0.001) and blood donation experience (β = 0.117, t = 2.687, p = 0.008) and these variables explained 56.9% (F = 152.610, p<0.001). In order to activate organ donation, it is necessary to form positive perspectives about organ donation in a long term perspective and to support altruistic activities such as blood donation.