Authors : Raju Mondal, Pranit Mukherjee, Kanti Meena, Sourav Dutta and Asit B. Mandal
Abstract: An attempt was made to develop a robust in-vitro regeneration protocol in mesta involving both the cultivated species-H. sabdariffa and H. cannabinus. The seed germination was found to be maximum (100%) in var. HS 4288 and minimum (80%) in var. HS 7910 in H. sabdariffa whereas in H. cannabinus maximum (90%) and minimum (76%) germination were observed in vars. MT 150 and AMC 108, respectively. Twenty three combinations of plant growth regulators (NAA, BAP, Kinetin, 2, 4-D, TDZ and IAA) in MS medium containing vitamins of B5 medium were assessedin governing in-vitro culture response involving cotyledon and hypocotyl explants. The best callus induction was observed in case of cotyledon explant. The maximum callus proliferation (97.8%) was noticed in-var. HS 4288 on MS containing 2 mg L–1 2, 4-D, 0.1 mg L–1 NAA and 2 mg L–1 Kinetin in H. sabdariffa. Whereas, in H. cannabinus maximum callus proliferation (66.8%) was observed in var. MT 150 on MS containing 0.8 mg L–1 IAA and 0.05 mg L–1 TDZ. The adjuvants showed good response in callus proliferation, however, no effect was observed on shootlet regeneration. The shootlet regeneration was found only in var. HS 4288 on MS medium containing 3.0 mg L–1 BA with 0.2 mg L–1 IAA and appreciable rooting on growth regulator free MS medium.
Raju Mondal, Pranit Mukherjee, Kanti Meena, Sourav Dutta and Asit B. Mandal, 2020. In-vitro Culture Response and Regeneration of Plantlets in Mesta. Plant Sciences Research, 12: 35-42.