Authors : Zhang Yong , Tang Hao-ru , Luo Ya , Jiang Hao and Hou Yanxia
Abstract: Two genes fragments from cultivated strawberry (Fragaria x ananassa cv. Toyonaka and cv. Akihime) genomic DNA were isolated by PCR amplification. The study showed that these genes were 1575 bp in length and contained a 988 bp Open Reading Frome (ORF) and 2 extron and 1 intron. The homology of nucleotide and deduced amino acid were 99.05 and 98.56%, respectively. Sequecing revealed that the 2 genes encoded a class chitinase. BLAST analysis showed that the homology of deduced amino acid between strawberry and other species’s CHI2 gene were about 50%. The homology tree showed that it obviously distinguished from the others.
Zhang Yong , Tang Hao-ru , Luo Ya , Jiang Hao and Hou Yanxia , 2009. Cloning and Sequencing of Strawberry Chitinase Gene CHI2. Research Journal of Biological Sciences, 4: 624-628.