Authors : Mohammad Karimian, Mehran Miroliaie, Kamran Ghaedi and Zohreh Zahraie
Abstract: Lipid Transfer Protein encoded by LTP gene in plants. This is a GC rich gene. These base causes that designed primers for LTP gene produce many secondary structures in annealing step of PCR and eventually does not produce a suitable product. Substances such as betaine, Dimethyl Sulfoxide (DMSO) and Ammonium Sulfate (AMS) can to eliminate this obstacle. In this study, the gradient of each material was placed in the PCR reaction and ultimately the optimum concentration for any of this material was obtained. In between, 4.5 μL betaine (5M), 2.25 μL DMSO (10%) and 2.25 μL AMS in the overall volume of 25 μL of PCR mixture were reported optimal concentrations.
Mohammad Karimian, Mehran Miroliaie, Kamran Ghaedi and Zohreh Zahraie, 2010. Improve the Amplification of LTP (Lipid Transfer Protein) Gene. Research Journal of Biological Sciences, 5: 802-804.