Authors : Marco Antonio Becerril Flores , Salazar Schettino Paz Maria and Ramirez Zamudio Lina
Abstract: To better understand the biological heterogeneity of behavior of Trypanosoma cruzi strains isolated from Mexico, 6 strains of T. cruzi were cloned by the Miles� method (drops of suspension with parasites were diluted in phosphate-buffered saline solution and inoculated to mice).Virulence and infectivity of 10 or 11 clones derived from each strain were determined in female Balb/c mice and Vero culture cells, respectively. Variability of clones was determined by Tukey F statistic test. Only 1 strain, T5, showed interclonal variability and its clones were subcloned by the same method; they showed similar behavior as their parental clones. Clones increased the virulence or had the same behavior after maintaining them for 1 year in mice. Seven clones were eliminated and the virulence of the 4 remaining clones was attenuated when they were maintained in LIT axenic culture for 1 year. The study showed that the strains of T. cruzi in Mexico are either monoclonal or polyclonal and the clones could be eliminated or selected from the environment along time; therefore it is possible to observe that the behavior of T. cruzi strains can change when they are maintained in laboratory for several years.
Marco Antonio Becerril Flores , Salazar Schettino Paz Maria and Ramirez Zamudio Lina , 2008. Analysis of Variability of Clones and Subclones of Trypanosoma cruzi Derived from Mexican Strains by the Behavior in Mice and Culture Cells. Research Journal of Medical Sciences, 2: 166-174.