Abstract: This study aimed at assessing the underlying causes of antimicrobial resistance in Saudi Arabia. About 50 patients randomly selected whose proved to have Staphylococcus antimicrobial resistance detected by Bact tech in Assir hospital microbiology laboratory. A special questionnaire was used to assess the knowledge, attitude and practice of patients towards using antimicrobials beside epidemiological and demographic data. Results showed that the nationality of all patients were Saudian (49 patients) only one was a Sudanese. About 22 patients were 16-40 years of age, the attitude of patients towards their treating doctor or pharmacist involved 13, deferent 11, critical (serious) 14 and ignored by 12 which almost equally distributed. About 64% patients used to exaggerate their symptoms to their doctor to let him prescribed any antimicrobial drugs. About 64% of patients purchase the antimicrobial drugs depending on the quality. Knowing the dosage of antimicrobial was enlisted by 84% patients. About 64% of patients used to have description of the antimicrobial dosage by treating physicians 20% by pharmacists. About 35 patients (70%) have received drug side effects description. The patients compliance grading <50% towards following their medication dosage was enlisted in 22 patients (44%). About 27 patients (54%) have no home animals while 23 patients (46%) have got home animals (7 patients have cats, 7 have goats, 6 have sheep, 2 have camels and 1 have dog). Only 7 patients (14%) have noticed infected wound on their home animals, 2 patients of them (4%) used to treat their home infected animals. About 20 patients (40%) used to through some of antimicrobial home waste in open carbidge. Conclusion deduced from of this study; antimicrobial resistance is a major health problem and patients often do not comply with antimicrobial prescription, enhancing the development of antimicrobial resistance during treatment.