Authors : I. Beishova, G. Chuzhebaeva, A. Kovalchuk, N. Kalimov, A. Stakheev and S. Zavriev
Abstract: Diagnosis of pathogenic species of fungi of the genus Fusarium has always been hampered by the confusing phenotypic classification system. We used a method based on quantitative PCR (real-time PCR) with primers and probes developed by our laboratory which allows to quickly and accurately identify three pathogenic species of Fusarium fungi common in Northern Kazakhstan: F. graminearum, F. culmorum and F. oxysporum. The method is based on PCR amplification of DNA fragments which were developed on the basis of the translation elongation factor gene 1-α (tef1α). In addition to providing accurate, reliable and rapid diagnosis of fungi of the genus fusarium in cereals, this method excludes the use of carcinogens (ethidium bromide). since, quantitative PCR uses fluorescent labeled probes, i.e., no gel electrophoresis is required.
I. Beishova, G. Chuzhebaeva, A. Kovalchuk, N. Kalimov, A. Stakheev and S. Zavriev, 2020. Diagnosis of Phytopathogenic Fungi of the genus Fusarium in Cereals on the Territory of the Republic of Kazakhstan using the Quantitative PCR Method. Research Journal of Medical Sciences, 14: 184-188.