Authors : I. Beishova, G. Chuzhebaeva, V. Ulyanov, Zh. Zharlygassov, G. Sultangazina, A. Stakheev and S. Zavriev
Abstract: Traditional diagnostic methods, such as enzyme-linked immunosorbent assays and microscopic diagnostics, require quite a lot of time do not have the necessary high sensitivity and do not always provide unambiguous identification of species. Therefore, the creation of test systems that allow the detection of pathogens quickly and accurately remains an urgent task. The Polymerase Chain Reaction (PCR) method is more sensitive and specific in comparison with microbiological methods and its real-time modification (quantitative PCR) using a hybridization probe with a fluorescent label makes it possible to quickly analyze the results without performing electrophoresis.
I. Beishova, G. Chuzhebaeva, V. Ulyanov, Zh. Zharlygassov, G. Sultangazina, A. Stakheev and S. Zavriev, 2020. Development of Sensitive High-Specific Rapid Tests Based on Dna Markers for the Diagnosis of Pathogenic Fungi of genera Puccinia and Pyrenophora, Causing Diseases of Cereals. Research Journal of Medical Sciences, 14: 84-88.