Authors : Elham Raeisi, Parisa Mousavi and Bahman Rahimi Esboei
Abstract: There is not enough information about the nosomiasis infection in Iran and also in other parts of the world. Moreover, the distinct genotype of each area in not fully determined, so, this study was aimed to detect microsporidians (N. apis and N. ceranae) of honeybee by and genotyping of the parasite using PCR-RFLP technique. During June to December, 2016, 180 adult honeybee samples were gathered from different parts of Northwest areas of Iran and nosomiasis was evaluated and genotyping by microscopic and PCR-RFLP technique and the results were confirmed by sequencing. About 75 samples out of 180 samples were microscopically positive for nosema spores. From 75 microscopically positive samples, 42 (56%) samples were positive by PCR amplifications and all were diagnosed as N. ceranae. Our results revealed that the N. ceranae opposite to the before believes is the main strain of Nosema in Northwest of Iran but future works with the larger sample size is recommended.
Elham Raeisi, Parisa Mousavi and Bahman Rahimi Esboei, 2020. Molecular Identification of Nosema apis and Nosema ceranae in Europian Honey Bee (Apis mellifera) in Northwestern of Iran. Research Journal of Animal Sciences, 14: 12-15.