Authors : A.E.A. Baragob, O.Y. Mohammed, A.E.A. Hana, H.A. Samia, S.M.E. Khojali and S.M. Alkarib
Abstract: The increasing number of diabetic patients in Sudan in addition to the large quantities of wasted camel pancreas in the slaughter houses stimulated the research in this project aimed at extracting, purifying and analyze camel insulin from the local sources. In this study, collected fresh camel pancreatic glands were trimmed small pieces then homogenized, centrifuged after adjustment of pH and then filtered. Crude insulin is precipitated from the filtrate and dissolved in acetic acid. Insulin was again precipitated iso-electrically and crystallized using zinc acetate. After the processes of extraction the processes purification, amino acid analysis and study of the presence and function in the camel insulin. The products of insulin scale were subjected to a wide range of quality control testes including yield percent, zinc content (mg/100 μ), nitrogen percent and purity test by electrophoresis amino acid analysis is a test to determine the quantities of each individual amino acid in camel insulin and study of the function groups of each amino acid.
A.E.A. Baragob, O.Y. Mohammed, A.E.A. Hana, H.A. Samia, S.M.E. Khojali and S.M. Alkarib, 2011. Extraction, Purfication, Drying and Analysis of Camel Insulin. Research Journal of Pharmacology, 5: 43-46.