Abstract: Gliomas are primary brain tumors that originate from the supportive cells of the brain. Gliomas are the most common tumors of the brain in adults, accounting for almost 80% of primary malignant brain tumors for which the most common type is (glioblastoma multiforme). In Iraq, brain tumors are the fifth most common tumor in adults and the second most common in children. Their incidence was 4.77% among males and 3.3% among females. Glioma invasiveness involves the attachment of tumor cells to the brain extracellular matrix, rich in Hyaluronic Acid (HA). CD44, the principal receptor for HA is a type transmembrane glycoprotein that was initially identified as a leukocyte antigen. CD44 is the major Hyaluronan (HA) receptor, its binding to HA has been proven to participate in various tumor biological activities including tumor progression, metastasis and proliferation, so, it can play a critical role in cell migration and extravasation. Assessment of CD44 expression in different types of gliomas. Correlation between CD44 scoring and patient’s age, sex and tumor grading. The reliability of using CD44 as diagnostic marker in gliomas. Forty cases of glioma where included in this study, they were collected from Al-Shaheed Ghazi Al Hariri Hospital in Baghdad medical city from January, 2016 through December, 2017. Their age ranging between (1-56) years with mean age of 31.4 year polymer based immunohisto chemichal technique was used for detection of CD44 antibody. Patients with grade I and IV were predominant the studied samples and composed each of 32.5% of whole samples while grade II and III represent 25, 10%, respectively. CD44 was positively expressed in 97.5% of cases but without significant statistical correlation with grade, age and sex. There is positive correlation between age and grade of tumors. From the above results we conclude that CD44 play important role in pathogenesis of glioma due to its high expression in alltumor grades but without positive correlation with histological types.