Authors : Muhammad Shuaib , Hamayun Khan , Muhammad Ashfaque and Sajid-ur-Rehman
Abstract: This research was carried out at the Department of Microbiology, University of Agriculture Faisalabad, Pakistan in order to study Macrophage Migration inhibition Factor produced by lymphocytes. The M1F activity of control samples without any plasma sample showed an average migration of macrophages with 410.23 �. Migration of macrophage inhibition activity in single vaccinated broiler chicks when compared with control macrophage migration, the macrophage migration inhibition% activity ranged from 14. 29 to 35.7% with the average of 28.57%. Further it was observed that maximum MIF activity in this group did not exceed to 35.7% 4 broilers showed 35.7%, 3briolers showed 28.57%, 2briolers showed 21.43% and one showed 14.29% MIF (%) activity in 10 broiler chicks. Migration of macrophage inhibition activity in boostered vaccinated broiler chicks when compared with control macrophage migration, it was investigated that ten-broiler chicks showed MIF per cent activity after double vaccination ranged from 28.57 to 42.86%. Further it was found that 3 broilers showed 28.57% 1 broiler showed 32.13%, 2 broilers with 35.7% and 4 broilers showed 42.86% MIF activity. The results obtained during present investigations revealed that Lasota vaccine strain of ND induces MIF 28.57% after 14 days of first vaccination and to obtain a better immune response, a double vaccination, 7 days after the single one is necessary.
Muhammad Shuaib , Hamayun Khan , Muhammad Ashfaque and Sajid-ur-Rehman , 2006. Cell Mediated Immune Response to Newcastle Disease Vaccine (Lastoa strain) in Chicks. Journal of Animal and Veterinary Advances, 5: 434-439.