Authors : H.B. Ciftci
Abstract: This study is presented to test the effect of injecting a mixture of Serine/Threonine or Glycine amino acids to determine and measure the changes in mRNAs expression for TGF 2, 3 within the mouse testes and the ovaries. Male (n = 30) and female (n = 30) mice (CD-1 strain) were injected, once a day for 5 days, with 0.2 mL Saline (control), 0.2 mL Saline with a mixture of Serine/Threonine (test) containing 0.26 ï¿½g L Threonine, 0.13 ï¿½g L Serine or 38 ng Glycine (Test). Total RNA was extracted and transferred on to a nylon membrane then probed with 32P-lebelled cDNA probes. The expression of mRNA for TGF 2 increased in male mice while there were no differences in female mice. This study shows that TGF 2, 3 are expressed both in the ovary and testis and their expressions were increased by the injection of Serine/Threonine or glycine.
H.B. Ciftci , 2008. Determinations of mRNAï¿½s For TGF 2, 3 in Mouse Testes and Ovaries After a Five days Injection With Glycine or a Serine and Threonine Mixture. Journal of Animal and Veterinary Advances, 7: 106-109.