Authors : A.M. Hammam, C.S. Whisnant, A. Elias, S.M. Zaabel, A.O. Hegab and E.M. Abu-El Naga
Abstract: This study was carried out to investigate the effect of two media and adding supplements or hormones to IVM medium on buffalo oocyte maturation rate. Oocytes were cultured either in TCM-199 or Ham`s F-10 with BSA or FBS with or without hormonal supplements (PMSG, FSH and E2) or βME, under defined conditions (38°C, 5% CO2, high humidity) for 22-24 h. The matured oocytes were fertilized in vitro by frozen thawed semen capacitated in either Brackett-Oliphant or TALP media. Maturation rate was not affected by media type, but was affected by the supplements added to the media. The maturation rates were 75.0±3.1, 68.0±2.4, 80.4±4.4, 73.8±3.4 and 67.5±3.1% for TCM-199 supplemented with FBS, BSA; FBS+PMSG; FBS+FSH and FBS+E2, respectively) and 78.4±3.2 and 66.7±2.6% for oocytes cultured in Ham`s F-10 supplement with FBS and BSA, respectively. βME added to TCM-199 yielded a higher maturation (95.2±2.9%, p<0.05). Using FBS gave better maturation than BSA, while PMSG, FSH or E2 had no effect on maturation rate. It is concluded that buffalo oocytes can be matured in TCM or Ham`s F-10 supplemented with FBS with best results obtained when βME is added to the media. TALP gave higher fertilization results (33.7±2.3%) compared to BO (15.5±1.2%) (p<0.05).
A.M. Hammam, C.S. Whisnant, A. Elias, S.M. Zaabel, A.O. Hegab and E.M. Abu-El Naga, 2010. Effect of Media, Sera and Hormones on in vitro Maturation and Fertilization of Water Buffalos (Bubalus bubalis). Journal of Animal and Veterinary Advances, 9: 27-31.