Abstract: The objective of this study was to investigate the effects of nisin and propolis on in vitro fermentation of a 60:40 forage:concentrate diet using the rumen simulation technique and to compare their effects with the antibiotic monensin (positive control). Nisin, propolis ethanol extract and monensin were added daily at 2 mg, 100 μL (contained active substances of 2 mg crude propolis) and 5 mg to the fermentation vessels, respectively. Monensin caused expected changes in fermentation patterns (a significant decrease in NH3-N concentration, acetate and butyrate production, protozoa counts and a significant increase in propionate production). Nisin and propolis did not cause beneficial effects on fermentation efficiency except NH3-N concentration. NH3-N concentration was reduced (p<0.05) by them. In conclusion, the results of this study indicate that nisin and propolis might be useful additives to decrease ruminal ammonia production and to improve nitrogen utilization by ruminants.
Hakan Oeztuerk, Bahri Emre, Vedat Sagmanligil, Ilksin Piskin, Ulvi Reha Fidanci and Mert Pekcan, 2010. Effects of Nisina and Propolis on Ruminal Fermentation in vitro. Journal of Animal and Veterinary Advances, 9: 2752-2758.