Authors : Jia Yao, Ming Zhang, Yu-Mei Feng, Si-Yi Chen, Wei Wang, Jie Wang and Song-Jia Lai
Abstract: Interferon-tau (IFN-τ) secreted by blastular trophectoderm was thought to be the primary signal for pregnancy recognition during bovine early pregnancy period after pregnancy recognition, progesterone dominated pregnancy maintaining. Moreover, Matrix Metalloproteinases-2 (MMP-2) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) was important for embryo implantation. The objectives of studies were evaluated whether IFN-τ and progesterone regulated MMP-2 and TIMP-2 expression in cultured bovine Endometrial Cells (bECs). In the studies, bECs were cultured in DMEM-Ham’s F12 medium with free-serum. These cultured cells were divided into 4 groups and cultured in four types culture medium: culture medium without IFN-τ and progesterone (Co group); culture medium including 100 ng mL-1 IFN-τ (IN group); culture medium including 200 nmol L-1 progesterone (Pr group); culture medium including 100 ng mL-1 IFN-τ and 200 nmol L-1 progesterone (I+P group). Cultured bECs were harvested and extracted total mRNA after being cultured for 1, 3, 6 and 12 h and then expression level of MMP-2 and TIMP-2 were assessed with fluorescence quantitative PCR. The results showed that MMP-2 expression level in Pr, IN and I+P group were extremely significantly higher than in Co group (p<0.01). MMP-2 expression level in IN and I+P group was significantly higher than in Pr group (p<0.05). MMP-2 expression level was no statistical difference between IN group and I+P group (p>0.05); TIMP-2 expression level in Pr, IN and I+P group was extremely significantly lower than in Co group (p<0.01) and TIMP-2 expression level was no statistical difference among Pr, IN and I+P group (p>0.05). The conclusion indicated progesterone and IFN-τ induced MMP-2 expression in vitro cultured bECs whereas, progesterone and IFN-τ suppressed TIMP-2 expression in vitro cultured bECs.
Jia Yao, Ming Zhang, Yu-Mei Feng, Si-Yi Chen, Wei Wang, Jie Wang and Song-Jia Lai, 2011. Effects of Interferon-Tau (IFN-τ) and Progesterone on Transcription Level of Matrix Metalloproteinases-2 (MMP-2) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) in Cultured Bovine Endometrial Cells. Journal of Animal and Veterinary Advances, 10: 1833-1840.