Authors : Wael K. Elfeil, Reham R. Abouelmaatti, Changjiang Sun, Wenyu Han, Xiaokun Li, Jisheng Ma, Liancheng Lei, Shanshan Liu, Yongjun Yang, Yu Wang, Mostafa Mandour, Mohamed Fawzy and Mohamed N. Shalaby
Abstract: Toll like receptor mediate immune response through recognition of Pathogen-Associated Molecular Patterns (PAMPs) thus play important roles in host defense. Little is known about the Avian Immune System structure and the majority of it based on chicken as a Universal Avian Model while recent researches showed that a lot of factor not common between chicken and other birds. This research aimed to identify and clone the duck TLR4 as a model to Anseriformes species, researchers success to clone a novel functional dTLR4 mRNA and deposit into the NCBI GenBank database where the mRNA consist of 2529 nucleotide and it showed 81% identity with chicken and 78% identity with zebra finch while showed 95% identity with goose. The translated amino acid showed that the gene consist of 843 amino acid and the transmembrane structure analysis reveal a novel unique LRR motifs in duck and absent in both chicken and zebra finch at the position 56-80 also showed that TIR domain consist of 146 amino acid not 145 like chicken and zebra finch.
Wael K. Elfeil, Reham R. Abouelmaatti, Changjiang Sun, Wenyu Han, Xiaokun Li, Jisheng Ma, Liancheng Lei, Shanshan Liu, Yongjun Yang, Yu Wang, Mostafa Mandour, Mohamed Fawzy and Mohamed N. Shalaby, 2012. Identification, Cloning, Expression of a Novel Functional Anas platyrhynchos mRNA TLR4. Journal of Animal and Veterinary Advances, 11: 1727-1733.