Abstract: To screen for the interaction of target proteins with the virB4 gene in bovine embryo trophoblast cells that were infected with Brucella bacteria. A cell culture for bovine trophoblasts was established. The cDNA library of trophoblast cells infected with the vaccine strain RB51 was constructed and a Saccharomyces cerevisiae expression vector containing the gene virB4 was synthesized. Target proteins which interacted with virB4 were screened through a yeast two-hybrid system. The results showed that the cDNA library of bovine trophoblast cells infected with the vaccine RB51 strain was successfully constructed. A recombinant plasmid pGBKT7-virB4 was cloned successfully into the expression vector Y187. Thirteen different types of proteins that interacted with virB4 were screened through a yeast two-hybrid assay.
Zhang Hui, Wang Pengyan, Wang Yuanzhi, Guo Qian, Li Chengyao and Chen Chuangfu, 2012. Interactions of Target Proteins with the virB4 Gene from Bovine Embryo Trophoblast Cells Infected with Brucella abortus Screened Through a Yeast Two-Hybrid Assay. Journal of Animal and Veterinary Advances, 11: 1793-1799.