Abstract: An increased histone acetylation is associated with more effective formation of DNA replication complexes (Trichostatin A, TSA), a histone deacetylase inhibitor is able to enhance the pool of acetylated histone. Researchers observed the changes of the Sheep Fibroblasts Cells (SFCs) and Sheep Cumulus Cells (SCCs) being treated with TSA, a stronger immunofluorescent signal for acH4K12 was detected in the 1 cell stage (p<0.05) also the developmental rates of cloned embryos was increased significantly (p<0.05) however, a higher acetylation level were observed in SCCs naturely, no significant effects on acetylation level and developmental rates of cloned embryos were detected after TSA treatment. Moreover, the acetylation distribution in the group of SFC-TSA cloned embryos resembles that in In Vitro Fertilisation (IVF) embryos but TSA had no ability to modify the acetylation state of SCCs cloned embryos similar with the IVF embryos, only a hyperacetylate pattern was detected which means a proper pattern of acetylation in cloned embryo is more prone to be reprogrammed than hyperacetylation.
Ya-Xin Yao, Wei-Jun Guan, Xiang-Chen Li, Xiao-Hua Su, Yue-Hui Ma and He-Ping Li, 2012. Trichostatin A Treatment on Two Types of Donor Cells for Somatic Cell Nuclear Transfer. Journal of Animal and Veterinary Advances, 11: 1812-1818.