Journal of Animal and Veterinary Advances

Year: 2013
Volume: 12
Issue: 6
Page No. 788 - 790

A Novel PCR-RFLP (Xba I) Polymorphism Analysis of the Bovine Calpastatin (CAST) Gene in Chinese Luxi Cattle

Authors : Dongying Yang

Abstract: Calpastatin (CAST) is a specific inhibitor of the ubiquitous calcium-dependent proteases-μ-calpain and m-calpain, found in mammalian tissues. In the coding region of the bovine CAST gene (CAST) the new nucleotide sequence polymorphism was found being a substitution G→C at position 962 nt (consensus sequence-GenBank AF281256). Consensus of bovine CAST sequence with that of human revealed that G→C substitution was located at position 1460 nt of exon 12. Computer analysis of the mutation showed the Ser→Thr substitution for CAST protein. The frequency of alleles C and G was 0.41 and 0.59, respectively. The message from these results is that CAST gene may probably be considered as a candidate marker for beef quality.

How to cite this article:

Dongying Yang , 2013. A Novel PCR-RFLP (Xba I) Polymorphism Analysis of the Bovine Calpastatin (CAST) Gene in Chinese Luxi Cattle. Journal of Animal and Veterinary Advances, 12: 788-790.

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