Abstract: This study was conducted to investigate the gap between clinical and serological approaches as well as those between bacterial isolation and PCR technique to identify ovine Caseous Lymphadenitis (CL) cases. In addition, the possibility of using x-ray imaging technique as a diagnostic tool to detect visceral CL lesions was evaluated. Clinical detection and serological identification by recombinant Phospholipase D ELISA (rPLD-ELISA) were conducted on 188 sheep belong to 9 mobile flocks at Kafrelsheikh governorate, Egypt. Clinical examination showed a 24 (12.76%) clinical cases. Abscessiation of head and neck lymph nodes were the most common findings. On the other hand, rPLD-ELISA showed the positivity of 64.89% of the examined sheep. Corynebacterium pseudotuberculosis (C. pseudotuberculosis), the causative bacterium of CL was isolated only from 5 (20.83%) clinical cases. Because of the low isolation rate, PCR was conducted on the 11 culture negative cases and showed a positivity percentage of 72.7%. X-ray radiography was conducted on an Algerian ewe which was suffering from multiple superficial lesions, chronic progressive emaciation and respiratory distress. Radiography had showed a pulmonary radio-opaque mass that was identified after PM examination to be CL lesion.
Atef F. Oreiby, Yamen M. Hegazy, Magdy H. Al-Gaabary, Salama A. Osman, Mohamed A. Marzok and Mohamed Abushhiwaa, 2015. Studies on Clinical Identification, Elisa, Bacteriological Isolation, PCR and X-Ray Radiography for Diagnosis of Ovine Caseous Lymphadenitis. Journal of Animal and Veterinary Advances, 14: 250-253.