Authors : Jing Xiaoqi, QU Cheng, XU Zhengyu, Song Xiaoyue, SHI Lei, LIU Jiaorong, Dong Shuwei, LIU Jinwang, QU Lei and ZHU Haijing
Abstract: To determine the correlation between the 14-bp insertion/deletion (InDel) polymorphism in the promoter of CMTM2 gene and the growth traits of Shaanbei white cashmere goats. Shaanbei white cashmere nannies population (n = 1105 including 621 bred goats and 484 adult goats) were chosen. The body weight and body measurements of the goats were measured and ear tissues were collected. After the genomic DNA were extracted, PCR amplification were conducted, agarose gel electrophoresis and DNA sequencing were carried out to detect the InDel mutation in the promoter of the CMTM2 gene. The correlation between the InDel characteristics and growth traits of cashmere goats was analyzed using SPSS 23.0 Software. In Shaanbei white cashmere goats’ population, three genotypes of II, ID and DD were detected at the mutation site of CMTM2 promoter. The frequency of allele “I” (0.863) is higher than that of allele “D” (0.137), this InDel mutation conformed to Hardy Weinberg equilibrium (p>0.05) and showed low polymorphism (PIC<0.25). The 14-bp InDel site in the promoter region was extremely significantly correlated with the body height, hip width and height at hip cross in adult goats (p<0.01); further association analysis results showed that this site was significantly related with body height and the height at hip cross (p<0.05) and extremely significantly related to the hip width (p<0.01) in Shaanbei white cashmere goat population. The 14-bp InDel in the promoter of CMTM2 gene is significantly related to the body height, hip width and height at hip cross of Shaanbei white cashmere goats. This site can be used for Marker Assisted Selection (MAS) in Shaanbei white cashmere goats. Also, it provided a theoretical and practical basis for the breeding of Shaanbei white cashmere goats.
Jing Xiaoqi, QU Cheng, XU Zhengyu, Song Xiaoyue, SHI Lei, LIU Jiaorong, Dong Shuwei, LIU Jinwang, QU Lei and ZHU Haijing, 2021. Correlation Analysis Between Polymorphism of CMTM2 Promoter and Growth Measurements of Shaanbei White Cashmere Goats. Journal of Animal and Veterinary Advances, 20: 134-141.