Authors : Yu- Ting Liao, Kuo- Hsiang Hung, I- Ti Mi, Ming- Ban Huang, Wei- Ling Huang and Tzen- Yuh Chiang
Abstract: Heparin sodium is the raw material of heparin, a blood coagulant commonly used in medical treatment. Heparin is clinically used to prevent blood vessel thrombosis. In the past, heparin sodium was mainly extracted from porcine intestines. Here, an acid/base methodology was modified and used to extract heparin sodium from broiler viscera. Trypsin was added to smashed chicken’s intestines at pH 8.5. D208 resin was then applied to adsorb heparin, followed by a process through a series of acid/base changes. Crude heparin sodium product, recovered by drying, was purified with permanganate oxidation followed by centrifuging. Final heparin products were harvested from the precipitation. From 0.2 kg raw materials of a mixture of chicken’s intestines, 23.80 g crude heparin was obtained, while 31.40 mg heparin was isolated and recovered after purification. Accordingly, 119.00 g crude heparin and 159.00 mg purified heparin can be harvested from 1 kg raw materials. The yield of heparin isolated from broiler intestines approximates that from porcine. This study indicates that chicken can serve a new source of pharmaceutical heparin, especially given an outbreak of African swine fever in Asia.
Yu- Ting Liao, Kuo- Hsiang Hung, I- Ti Mi, Ming- Ban Huang, Wei- Ling Huang and Tzen- Yuh Chiang, 2021. Technical Notes: Heparin Sodium Extraction from Broiler Intestines Based on Acid/Base Methodology. Journal of Animal and Veterinary Advances, 20: 185-188.