Abstract: This proposed research we suggest sterilization method depend on laser system with new three different wavelengths the laser effect on milk content from amino acid was investigated. The sterilization process by laser system used new three wavelengths 540, 800 and 1100 nm at laser power 70 mw and hold time 1.5 min was achieved. All the test samples was analyzed by using amino acid analyzer where all results show no significant difference with respect to control sample. All test samples with 800 nm show standard amino acid and essential amino acid while when we use 800 nm we found standard amino acid and essential amino acid, laser system at wavelength 540 nm produce the optimum results where the results show the good results with essential amino acid this enhancement in milk content as a result of photochemical effect in visible region.