Authors : Haifa Sebii, Sirine Karra, Ines Gafsi Makhlouf, Sabine Danthine, Christophe Blecker, Hamadi Attia, Souhail Besbes and Brahim Bchir
Abstract: The present study investigates a comparison of two methods of extraction of date palm pollen protein. Two obtained pollen protein concentrates using the isoelectric precipitation and the ultrafiltration methods, (PPCIP and PPCUF) were obtained. From a physical point of view, PPCIP was a crystalline powder whereas PPCUF had an amorphous structure. The surface properties of the two protein concentrates varied according to pH and concentration. Indeed, it turned out that at pH 7, the two extracts were more capable of reducing the surface tension than at pH 4. Findings also proved that the surfactant proteins had a molecular mass >10 KDa. In addition, the solubility values were greater at pH 7 than at pH 4. These results were in line with the isoelectric points determined in the ZETA potential study which gave a pHi around 3 for the two extracts.The differential calorimetric analysis revealed that PPCIP had two denaturation peaks that could be attributed to the different constituents contained in the extract whereas PPCUF presented only one peak relative to the denaturation of DPP proteins. This difference was simultaneously noticed in the Thermogravimetric analysis.
Haifa Sebii, Sirine Karra, Ines Gafsi Makhlouf, Sabine Danthine, Christophe Blecker, Hamadi Attia, Souhail Besbes and Brahim Bchir, 2021. Effect of Isoelectric Precipitation and Ultrafiltration Treatment on Extraction and Physicochemical Properties of Proteins from Date Palm Pollen. Journal of Engineering and Applied Sciences, 16: 103-110.