Authors : Godratollah Mohammadi and Adel Saberivand
Abstract: DNA isolation from cells is the 1st and the foremost step involved in molecular biology. The high quality and purity of extracted DNA and simplicity as well as low cost of the extraction method is an important consideration for a molecular biologist. Genomic mammalian DNA is very long and extraction must avoid high levels of centrifuge and severe shakes. There are several methods to extract DNA, including: Boiling, Salting out, Phenol-Chloroform, Isopropanol Precipitation methods. In this study, a method which seems to be superior to salting out and other methods has been developed. The extraction of DNA by this method is carried out from leukocytes. This modified method is simple, cheap and has a wider application than other methods.
Godratollah Mohammadi and Adel Saberivand , 2009. Simple Method to Extract DNA from Mammalian Whole Blood. Journal of Molecular Genetics, 1: 7-10.