Abstract: Today, identity is a competitive advantage for organizations that have become important in recent years. Positive identity in an organization can prevent the occurrence of a phenomenon called isolation of internal staff. The aim of this study was to evaluate the effect of spirituality on work of organizational identity, according to the mediator’s work ethic in staff Amir Kabir Technical University. The goal of quantitative and fundamental research. The strategy of this research is descriptive and correlational. The population of the research staff of the university of Amir Kabir University which enjoyed 647 tons. The sample size of the study sample of 242 self-help measure and research the questionnaire milliman three standard questionnaire (2003) to measure spirituality in the standard questionnaire Gregory C. Petty to assess the work ethic and the standard questionnaire Ashforth and Miles used to measure organizational identity to help researchers construct validity and face validity of the questionnaire obtained. Also according to Cronbach’s alpha coefficient above 0.7, reliability was confirmed. Then with the structural equation LISREL Software test the research hypothesis. The results showed that spirituality at work is in a significant relationship with organizational identity. The research ethic of the organization as a mediator of the relationship between spirituality and work with organizational identity will have a significant effect. The dimensions of spirituality at work have a significant relationship with organizational identity and work ethic of the organization as a mediator in the relationship between spirituality at work and organizational identity puts a significant effect.