Authors : Asma Saleh W. El-Mabrok, Zaiton Hassan, Ahmed Mahir Mokhtar, Khaled M.A. Hussain and Mohamed Mustafa Aween
Abstract: The objective of this study was isolation, identification of lactic acid bacteria from different source and testing their antifungal activity against phytopathogens fungi C. capsici and C. gloeosporioides. The isolates were identified by their phenotypic and genotypic characteristics and their antifungal activity was performed by the overlay method. Seven LAB isolates were showed good antifungal activity against phytopathogens fungi and were grew at different temperatures, pH and NaCl. However, C5 and G7 have the ability to inhibit the growth of both target phytopathogens fungi compared to other isolates. C5 and G7 identified by API 50CHL kit as Lactobacillus plantarum and the identified by used 16S rDNA was Lactobacillus plantarum C5 and L. pentosus G7. This is the first study that observed LAB isolates L. plantarum C5 and L. pentosus G7 inhibited both of C. capsici and C. gloesporeioides in vitro. This study demonstrates that the LAB L. plantarum C5 and L. pentosus G7 have potential to be used as biological control of this phytopathogen by inhibiting the mycelia growth.
Asma Saleh W. El-Mabrok, Zaiton Hassan, Ahmed Mahir Mokhtar, Khaled M.A. Hussain and Mohamed Mustafa Aween, 2012. Isolation and Identification of Lactic Acid Bacteria with Antifungal Activity Against Anthracnose Disease. Research Journal of Biological Sciences, 7: 335-339.