Abstract: Peripheral Blood Mononuclear Cells (PBMCs) are key regulators of inflammation. Vascular Endothelial Growth Factor (VEGF) is a cytokine which is produced by a number of cells and has an important role in inflammation. In this study, the patterns of VEGF expression by human PBMCs were assessed in vitro using Phytoheamagglutinin (PHA), Phorbol Myristate Acetate (PMA) and Lypopolysaccharide (LPS) as VEGF inducers. Human PBMCs were cultured in complete RPMI-1640 medium. Then, the cells were seeded at a density of 10⁶ cells mL^-1 and subsequently were incubated with PMA (0-25 ng mL^-1), PHA (0-10 μg mL^-1) or LPS (0-4 μg mL^-1) for 48 h. The level of VEGF secreted in the cell culture supernatants was measured with enzyme-linked immunosorbent assay kits (R and D systems). Human PBMCs cultured without any stimulus, produced detectable amount of VEGF after 48 h incubation. PMA significantly reduced while PHA/LPS significantly increased VEGF production in human PBMCs dose-dependently. The results show that PMA, PHA and LPS have different effects on VEGF production by PBMCs.