Authors : M. Razzaghi Maensh, S. Mahabadi, A. Ghamesi and A.R. Namjoo
Abstract: The present study was to identified a incidence of visceral leishmaniasis in owned dogs using Polymerase Chain Reaction (PCR) in Ardestan district of Isfahan. In this cross-sectional study, 184 owed dogs was sampled randomly. To determine the prevalence of visceral leishmania infection in dogs was used the Direct Agglutination Test (DAT) and Polymerase Chain Reaction (PCR). In this study, plasma samples from 184 dogs were examined using the direct agglutination. A total of twenty dogs (8.10%) have had positive titers. Four dogs (4.2%) of asymptomatic dogs and 16 dogs (8.8%) of dogs with symptoms had positive serum. Also statistically significant between visceral leishmaniasis with impotence, hair loss was seen (p<0.05). By using PCR, KDNA leishmania parasite identification and species of L. infantum was determined. The study results showed that peripheral blood by PCR with primers RV1 and RV2 on methods for early detection of disease in humans and dogs and also all cases are asymptomatic especially in endemic areas.
M. Razzaghi Maensh, S. Mahabadi, A. Ghamesi and A.R. Namjoo, 2012. Prevalence of Canine Visceral Leishmaniasis in Dogs at Adrestan District Detected by PCR. Veterinary Research, 5: 22-25.