Authors : Yourong Ye, Jie Sun, Shuang Li, Zongsheng Zhao, Daquan Li and Jinfu Wang
Abstract: Researchers investigated the effects of Estrogen (E2) and Progesterone (P4) on the morphology, growth and proliferation of chicken endometrial cells in vitro. Type I collagenase digestion was used to isolate endometrial epithelial and stromal cells and the cells were identified by immunohistochemistry. MTT assays (MTT, [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]) were performed to analyze the effects of the sex hormones on the proliferation of the endometrial cells in vitro and growth curves were constructed to demonstrate the growth rules of endometrial cells cultured in vitro. The epithelial and stromal cells tested positive for keratin CK18 and vimentin, respectively, in the immunohistochemical analysis. The MTT experiments showed that the growth rate of the chicken endometrial cells began to increase logarithmically on day 2, reaching the plateau phase on day 6 or 7. The endometrial epithelial cells treated with 100 nM E2 and/or 100 nM P4 proliferated at a significantly higher rate p<0.05) than the control cells. However, although, the stromal cells treated with 100 nM P4 proliferated at a significantly higher rate (p<0.05) than the control cells, the E2-treated endometrial stromal cells did not.
Yourong Ye, Jie Sun, Shuang Li, Zongsheng Zhao, Daquan Li and Jinfu Wang, 2014. In vitro Effects of Sex Hormones on the Proliferation of Endometrial Cells Isolated from Laying Hens. Veterinary Research, 7: 13-19.