Authors : Hadi Haghbin Nazarpak, Nariman Sheykhi, Alireza Dibaei1 and Yones Goleij
Abstract: The light is an important environmental factor in industrial poultry husbandry. The light programs play an important role in appropriate management and an infrastructure for desired function of broilers. In this study, the effect of various light programs on heterophil to lymphocyte ratio and on antibody titer against Newcastle disease with hemaglutination inhibition test was evaluated. In addition, 800 one-day ROSS 308 broiler chicken were prepared and randomly divided into four groups, each group contained 200 chicken, nominated as A-D. The groups kept at controlled isolated locations, containing two different light programs. Group A was adjusted on 23 h lighting and one hour darkness and group B was designed as recommended program for Ross breed (2-3 kg end period body weight). Although, group C and D were considered as control groups and did not receive Newcastle vaccine. During husbandry period the groups were in same conditions and just were different in lighting programs. The groups A and B on 8, 18 and 29 days were vaccinated against Newcastle disease and on 1, 7, 14, 21, 35, 28 and 42 day old the blood samples were taken for blood smears for heterophil to lymphocyte ratio count and antibody titer assessment with hemaglutination inhibition test. The obtained data were analyzed with t-test method. The results showed differences of heterophil to lymphocyte ratio between A and B groups on 7, 14 and 42 day old which the ratio was significantly higher in group A than group B (p<0.05). On 21 and 28 days the significant difference in this ratio was not observed (p>0.05). On 35 day-old higher in group B than group A (p<0.05). Furthermore, the antibody titer of Newcastle vaccine showed a relative priority in group B on 7, 14, 21, 28, 35 and 42 days than group A whereas no significant difference were observed in any days (p>0.05). The results indicated stress condition in group A, containing lighting program for Ross husbandry (2-3 kg end body weight) than group B.
Hadi Haghbin Nazarpak, Nariman Sheykhi, Alireza Dibaei1 and Yones Goleij, 2015. The Effect of Various Light Programs on Heterophil to Lymphocyte Ratio and on Antibodytiter Against Newcastle Disease. Veterinary Research, 8: 32-36.