Authors : Sakine Ülküm Çizmeci, Dursun Ali Dinç, Mustafa Numan Bucak, Muhammed Furkan Çiftçi, Ömer Faruk Yeşilkaya, Vahit Ağir, Ayşe Sari, Maide Gölbaşi and Hasan Ali Çay
Abstract: This study was aimed to produce in-vitro embryos massively and at a lower cost. For this purpose, it was aimed to determine and compare the blastocyst growth rates of oocytes collected from slaughterhouse and by Ovum-Pick up (OPU) method. The animal material to be used in the OPU application of the study consisted of 10 Holstein breed heifers 15-25 months-old. A total of 51 times OPU was applied to the animals. In the postmortem study, 52 ovaries belonging to 26 Holstein cows obtained from local slaughterhouses were used. After the obtained oocytes were evaluated according to their quality, they were taken to maturation, fertilization and culture stages respectively. In the presented study, total of 359 oocytes were collected in 51 OPU applications. The cleavage rate was 85.07% and the rate of blastocyst growth was 24,63%. Collected from the slaughterhouse, 325 oocytes were obtained from 52 ovaries. The cleavage rate was determined as 94.19% and the rate of reaching blastocyst was determined as 25.73%. As a result, it was observed that there was no statistical difference in blastocyst development rates (p>0.05). In the production of in vitro embryos, it has been concluded that by obtaining post-slaughtering ovaries of females with high genetic value, the loss of genetic material can be reduced by producing their embryos at cheaper costs (cost-effective). It is suggested that in-vitro embryo production can be carried out massively and at a cheaper cost from slaughtered female animals and material with average or above genetic characteristics can be evaluated.
Sakine Ülküm Çizmeci, Dursun Ali Dinç, Mustafa Numan Bucak, Muhammed Furkan Çiftçi, Ömer Faruk Yeşilkaya, Vahit Ağir, Ayşe Sari, Maide Gölbaşi and Hasan Ali Çay, 2021. Evaluation of Blastocyst Rates of Immature Bovine Oocytes Collected by Ovum Pick up (OPU) and Slaughterhouse. Veterinary Research, 14: 27-33.